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Abteilung fÃ¼r Zellbiologie (Anatomie III)
82152 Planegg Martinsried
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In mature hippocampal neurons, a subset of RNAs localizes to dendrites within ribonucleoprotein particles (RNPs). These RNPs not only contain localized mRNAs and RNA-binding proteins (RBPs), e.g. the brain-specific double-stranded RNA-binding protein Staufen2 (Stau2), Barentsz (MLN51, Casc3) and Pumilio 2 amongst others, but also a set of non-coding mRNAs. Together with RBPs known to regulate translation, these miRNAs are crucial to keep mRNAs in a translationally repressed state during transport to the synapse. In this project, we aim to identify non-coding mRNAs in Stau2 and Pum2 RNPs and study the underlying mechanism of translational control occurring at individual synapses of hippocampal neurons. In conclusion, we hope to understand how local protein synthesis contributes to morphologically and functionally reorganize individual synapses upon learning and memory.
We would be happy to help with :
- FISH in neurons
- MS2 RNA imaging in neurons
Kusek G, Campbell M, Doyle F, Tenenbaum S, Kiebler MA, Temple S (2012). Segregation of the double-stranded RNA-binding protein Stau2 during mammalian asymmetric neural stem cell division promotes lineage progression and differentiation. Cell Stem Cell 11, 505-16.
Fritzsche R, Karra D, Bennett K, Ang Fy, Heraud-Farlow J, Tolino M, Doyle M, Bauer KE, Thomas S, Planvavsky M, Arn E, Bakosova A, Buhlert K, HÃ¶rmann A, Palfi Z, Sandholzer J, Schwarz, M, Macchi P, Colinge J, Superti-Furga G. and Kiebler MA (2013) Interactome of two diverse RNA granules links mRNA localization to translational repression in neurons (Resource). Cell Reports 5:1749-62.
Tuebing F, Vendra G, Mikl M, Macchi P, Thomas S, and Kiebler MA. (2010) Dendritically localized transcripts are sorted into distinct RNPs that display fast directional motility along dendrites of hippocampal neurons. J. Neurosci. 30:4160-70.
Konecna A, Heraud JE, SchoderbÃ¶ck L, Raposo AASF, and Kiebler MA (2009). What are the roles of microRNAs at the mammalian synapse? Neuroscience Letters (special issue: Non-coding RNAs), 466, 63-8.
Schratt G, Tuebing F, Nigh EA, Kane C, Sabatini MW, Kiebler MA and Greenberg ME (2006). A brain-specific microRNA regulates dendritic spine development. Nature 439, 283-289.